Dr. Hanspeter Saluz, Dr. Jean-Pierre Jost (auth.)'s A Laboratory Guide to Genomic Sequencing: The Direct PDF

By Dr. Hanspeter Saluz, Dr. Jean-Pierre Jost (auth.)

ISBN-10: 3034893027

ISBN-13: 9783034893022

ISBN-10: 3764319259

ISBN-13: 9783764319250

Show description

Read or Download A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA PDF

Best nonfiction_10 books

Foundations and Applications of Decision Theory: Volume I - download pdf or read online

1. creation within the Spring of 1975 we held a world workshop at the Foundations and alertness of selection idea on the college of Western Ontario. to assist constitution the workshop into ordered and attainable periods we distriĀ­ buted the subsequent assertion of our pursuits to all invited individuals.

Download e-book for kindle: Endocrine and Metabolic Disease by Colin M. Feek MBBS, MRCP, Christopher R. W. Edwards MD,

Endocrine and Metabolic illness is in sections. the 1st is a scientific overview of scientific and healing features of endocrine illnesses, while the second one considers the medical pharmacology of substances utilized in endocrinology. all of the significant components of endocrinology and metabolism are coated. Thyroid, adrenal and pancreatic affliction are mentioned intensive and there are separate chapters at the ovary and testes in addition to at the pituitary gland and parathyroids.

Quantitative Methods in Bone Densitometry by Alan Huddleston PDF

Curiosity in bone densitometry tools has lately skilled a resurgence in the scientific group. Physicians became extra than ever ahead of within the prognosis and remedy of degenerative ailments of bone comparable to osteoporosis. the general public belief of osteoporosis and its prevention has been lately heightened.

Download e-book for kindle: The Kidney and Hypertension in Diabetes Mellitus by Bruce L. Riser, Pedro Cortes (auth.), Carl Erik Mogensen

Few problems of systemic ailments are larger understood than diabetic nephropathy. largely, growth during this zone is because of Carl Erik Mogensen's steadfast preoccupation over greater than 3 a long time with the disorder's epidemiology, pathogenesis, pathophysiology, scientific analysis and evolving innovations of administration.

Additional info for A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA

Sample text

Add 750 JLl of precooled ethanol, mix thoroughly by inversion, chill at -700 C and centrifuge as described above. > To wash the pellet, add carefully 1 ml of 70 % ethanol/water, centrifuge for 5 minutes and carefully pour out the supernatant and centrifuge again for 1 minute. > Remove the residual ethanol with a drawn out glass capillary and dry the pellet in the speed vac. > The DNA is ready for piperidine treatment (see at the end of the chapter). LI of water by tapping the tube. LI of formic acid

The number of cuts per target DNA molecule can greatly affect the resolution of the genomic sequence. If at least one of the two cuts is located within the probe region, for example, two or three hybridizable fragments are created. Such fragments result in additional unspecific bands within the sequencing lanes and makes the sequence more difficult if not impossible to interpret (see 'Trouble-Shooting Guide': Fig. 2112). --J<-.. 4. T-specific reaction Comparison ofG- and T -specific reactions of potentialmethylation sites (b, c, d) in the upstream area (upper strand) of the avian vitellogenin gene II (L = liver DNA, E = erythrocyte DNA.

The genomic DNA was digested overnight at the temperature recommended by the supplier and with a three-fold excess (units/JLg DNA) of restriction enzymes (6 basepair cutters) and a ten-fold excess (units/JLg DNA) for 4 base-pair cutters. Following digestion, the genomic DNA should be treated at least once by phenol extraction. After the digestion with restriction enzymes it is routine to perform at least one classical Southern-blot analysis (Southern, 1975) to check that 1) the DNA was digested to completion and 2) that there is only one DNA frag- INTRODUCTION 33 ment, hybridizing with the radioactively labeled probe.

Download PDF sample

A Laboratory Guide to Genomic Sequencing: The Direct Sequencing of Native Uncloned DNA by Dr. Hanspeter Saluz, Dr. Jean-Pierre Jost (auth.)


by George
4.2

Rated 4.27 of 5 – based on 47 votes

About the Author

admin