By Karl Maramorosch, Frederick A. Murphy
The first assessment sequence in virology and released considering 1953, Advances in Virus Research covers a various diversity of in-depth stories, supplying a important evaluate of the sphere.
- Contributions from top authorities
- Comprehensive stories for normal and professional use
- First and longest-running evaluate sequence in virology
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Additional resources for Advances in Virus Research, Volume 88
To reduce these technical limitations, a set of minimal requirements for RNAi screens have been established. This includes a rigorous validation process based on two or more siRNAs targeting different sites in the mRNA of interest and showing the same phenotype. , 2005). 2. Protein interaction networks A complementary approach to identify host factors involved in a viral replication cycle is the analysis of protein interaction networks. Two methods Revisiting Dengue Virus–Host Cell Interaction 29 that are frequently used are based on high-throughput yeast two-hybrid assays or coaffinity purification followed by mass spectrometry.
2003). , 2006). , 1994). , 2003). Experiments designed to decipher the mode of action of DC-SIGN for DENV entry revealed that this factor is dispensable for virus internalization. , 2003). Thus, DC-SIGN acts as a DENV attachment factor, which might facilitate the interaction of virus particles with a cellular receptor responsible for internalization. , 2006). , 2005). , 2011). Two independent studies of patient cohorts from Thailand and Taiwan show a strong association with genotypes 42 Eliana G.
Among them, heparan sulfate proteoglycan (HSPG) and nucleophosmin showed decreased abundance, whereas superoxide Revisiting Dengue Virus–Host Cell Interaction 33 dismutase, cyclophilin A, and a-enolase amounts were enhanced. Interestingly, a-enolase is known to serve as a strong plasminogen receptor and activator. , 2008). Proteome analyses have also been conducted with mosquito cells. , 2011). , 2011). , 2012). , 2009). , 2012). One aspect that has often been ignored in the past is the dependency of viruses on distinct host cell lipids.
Advances in Virus Research, Volume 88 by Karl Maramorosch, Frederick A. Murphy